麥康凱瓊脂培養(yǎng)基

更新時(shí)間：2016-06-11

簡要描述：

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麥康凱瓊脂培養(yǎng)基

MacConkey Agar

250g

用于腸道致病菌的選擇性分離培養(yǎng)。

用途：用于腸道致病菌的選擇性分離培養(yǎng)

麥康凱瓊脂培養(yǎng)基配方：（g/L）

蛋白胨
乳糖
牛膽鹽
氯化鈉
中性紅
瓊脂
PH值

20.0g
10.0g
5.0g
5.0g
0.03g
14.0g
7.1±0.2

原理：

蛋白胨提供碳氮源、維生素和生長因子；牛膽鹽可抑制革蘭氏陽性菌的生長；氯化鈉維持均衡的滲透壓；瓊脂是培養(yǎng)基的凝固劑；乳糖為可發(fā)酵的糖類；中性紅是pH指示劑，細(xì)菌發(fā)酵乳糖產(chǎn)酸時(shí)菌落呈粉紅色并在菌落周圍出現(xiàn)膽鹽沉淀的渾濁圈。

用法：

稱取本品54克,加熱溶解于 1000ml蒸餾水中，121℃高壓滅菌15分鐘備用。

質(zhì)量控制：

在36±1℃培養(yǎng)18-24小時(shí)。

質(zhì)控菌株	菌株編號	生長情況	其它特征
金葡萄球菌	ATCC25923	-	/
大腸埃希氏菌	ATCC25922	+++	紅色菌落、有膽鹽沉淀環(huán)
沙門氏菌	ATCC14028	+++	無色大菌落

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