更新時(shí)間:2016-06-16
人急性T淋巴細(xì)胞白血病細(xì)胞;Jurkat, Clone E6-1,開(kāi)始培養(yǎng)保持細(xì)胞濃度在2×10 exp5個(gè)細(xì)胞/ ml和1×10 exp5和1×10 exp6個(gè)細(xì)胞/ ml之間。只用于實(shí)驗(yàn)室研究。不能被用于在臨床檢測(cè)和有關(guān)于人身安全的實(shí)驗(yàn)。
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人急性T淋巴細(xì)胞白血病細(xì)胞;Jurkat, Clone E6-1
形態(tài)特性 淋巴母細(xì)胞樣
生長(zhǎng)特性 懸浮生長(zhǎng)
特征特性人急性T淋巴細(xì)胞白血病細(xì)胞;Jurkat, Clone E6-1該細(xì)胞是Jurkat-FHCRC細(xì)胞株(Jurkat細(xì)胞株的衍生)的一個(gè)克隆。Jurkat細(xì)胞株來(lái)源于一個(gè)14歲男孩的外周血;經(jīng)佛波酯和外源凝集素或抗T3單克隆抗體中的一種誘導(dǎo)后可產(chǎn)生大量IL-2(IL-2的產(chǎn)生需兩種類型的誘導(dǎo)劑);表達(dá)T細(xì)胞受體、CD3。
培養(yǎng)條件 RPMI 1640 (w/o Hepes) 10%FBS
傳代方法 維持細(xì)胞濃度在1×105~1×106viable cells/ml;2~3天換液1次?!?br />傳代情況 C5
凍存條件 基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS
公司細(xì)胞廣泛用于國(guó)內(nèi)院校的細(xì)胞生物學(xué)研究,他們的很多論文都或多或少使用我們公司的細(xì)胞,作為實(shí)驗(yàn)項(xiàng)目研究
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